This tab calculates the 'humanness' of your sequence using the method of Abhinandan and Martin (2007) [J. Mol. Biol 369:852-862].
The graph shows a distribution of scores for expressed human sequences in green and for expressed mouse sequences in blue. The score for the sequence of interest is indicated by the vertical red line.
The overall score for your sequence is shown below the graph and a link is provided to download the graph.
A score of zero represents a sequence with average humanness - positive scores are more representative of human sequences than average while negative scores are less representative of human sequences than average.
You should not normally need to change the options at the top of the graph.
The Comparison database dropdown allows you to select the dataset against which to compare the sequence. (The default is that the sequence is compared against all datasets and results are displayed for the best scoring dataset.)
The Display Mouse Distribution checkbox controls whether the graphs show the mouse distribution as well as the human distribution.
In brief, the distributions are created by scanning every human sequence in turn against all other human sequences and calculating the mean sequence identity to other human sequences (the 'raw humanness'). The mean of these raw humanness scores is then calculated and each sequence is then given a final humanness score expressed as a number of standard deviations away from the mean. Mouse sequences are compared against the human library in the same way.
The sequence of interest is also scanned against the library of human sequences and a score calculated in the same way.